Data are available via ProteomeXchange with identifier PXD026218. Most proteins, including TcdA, TcdB, flagellin subunit, and cell surface protein, were overrepresented in the ICC-45 strain 14 proteins, including mature S-layer protein, were present in higher proportions in LIBA5756. These proteins were involved with catalytic and binding functions and indirectly interacted with proteins related to pathogenicity. Only five proteins were exclusive to the ICC-45 strain. Exoproteome analysis revealed 197 proteins, of which 192 were similar in both strains. Only 83 and 290 NAP1/027 genes were considered singletons in ICC-45 and NAP1/027, respectively. Genomic analysis revealed that these isolates shared most of the gene composition. difficile isolates were cultured and extracellular proteins were analyzed using high-performance liquid chromatography-tandem mass spectrometry. difficile strains considered hypervirulent: ICC-45 (ribotype SLO231/UK821), isolated in Brazil, and NAP1/027/ST01 (LIBA5756), isolated during a 2010 outbreak in Costa Rica. We examined the genomes and exoproteomes of two multilocus sequence type (MLST) clade 2 C. Clostridioides difficile BI/NAP1/ribotype 027 is an epidemic hypervirulent strain found worldwide, including in Latin America.
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